Journal: Annals of Laboratory Medicine

Article Title: Development of a Rapid Automated Fluorescent Lateral Flow Immunoassay to Detect Hepatitis B Surface Antigen (HBsAg), Antibody to HBsAg, and Antibody to Hepatitis C

doi: 10.3343/alm.2018.38.6.578

Figure Lengend Snippet: Comparison of anti-HCV assays using commercial seroconversion panels

Article Snippet: We used 20 commercially available seroconversion panels to evaluate the sensitivity of the HBsAg (HBV6285, HBV11005, HBV6293, HBV6273, HBV6287, HBV11002, HBV11004, HBV11059, HBV11064, and HBV9072; ZeptoMetrix Co., New York, NY, USA) and anti-HCV (HCV6226, HCV6228, HCV9041, HCV9044, HCV9046, HCV9047, HCV10071, HCV10165, HCV10185, and HCV10235; ZeptoMetrix Co.) tests.

Techniques: Comparison

Journal: ACS sensors

Article Title: Compact Point-of-Care Device for Self-Administered HIV Viral Load Tests from Whole Blood

doi: 10.1021/acssensors.3c01819

Figure Lengend Snippet: Overall Device Workflow and Assay Design. (a) An image showing the components of the device, including 1. an Android phone, 2. a syringe-based RNA extraction module, 3. an analyzer, and 4. a lancet. (b) The procedure, as comprehensively delineated in the mobile application, involves a user initiating the app, self-collecting a blood sample using a disposable pipette and plasma separation card, transferring the absorbent paper into a pre-filled lysis buffer collection tube, conducting RNA extraction, introducing the eluted RNA and water into the testing tube, and finally, after 40 minutes, receiving the test results via the APP. (c) The design of primers. This includes a set of 6 RT-LAMP primers that target the medium region of the pol gene. (d) Real-time HIV-1 lyophilized RT-LAMP amplification data preserved when reconstituted from powder with serially diluted HIV RNA standards (six repeats per concentration). (e) Duration to positive value at various HIV-1 RNA concentrations; defined as the time for the RFU to hit a 1000 threshold (dashed line in d). (f) Results of HIV-1 lyophilized RT-LAMP real-time amplification over 16 weeks, replicated three times weekly using PCR-grade water and lyophilized RT-LAMP powder, plus 1000 HIV-1 RNA copies. (g) Data showing HIV-1 lyophilized RT-LAMP real-time amplification performance within a 16-week period. Each reaction was replicated three times per testing week using only PCR-grade water and lyophilized RT-LAMP powder (containing 1000 copies of HIV-1 RNA) mixed together. (h) The results of a test evaluating the shelf-life of lyophilized HIV-1 RT-LAMP.

Article Snippet: HIV-1 RNAs were from SeraCare, while blood samples were from Innovative Research.

Techniques: RNA Extraction, Transferring, Lysis, Amplification, Concentration Assay

Journal: ACS sensors

Article Title: Compact Point-of-Care Device for Self-Administered HIV Viral Load Tests from Whole Blood

doi: 10.1021/acssensors.3c01819

Figure Lengend Snippet: The analyzer and its performance for RT-LAMP amplification and detection for HIV semiquantitative self-testing. (a) Renderings showcase a fully enclosed analyzer along with a partially exploded view (dimensions: 88×68×82 mm; weight: 200 g). (1) Users activate the analyzer and initiate detection reactions via push buttons, with real-time updates provided by the status indicator. (2) The analyzer is Bluetooth-enabled for data transmission. Optical, thermal, and electromagnetic array subsystems are seamlessly integrated, supporting streamlined nucleic acid testing. The entire analyzer can be powered by an internally rechargeable battery. (b) This panel presents real-time RT-LAMP data from a single-analyzer test with serially diluted HIV-1 RNA. Each concentration was tested in triplicate. (c) This panel depicts the extracted “Time to positive” value from the single-analyzer test.

Article Snippet: HIV-1 RNAs were from SeraCare, while blood samples were from Innovative Research.

Techniques: Amplification, Transmission Assay, Battery, Concentration Assay